Holders Valletta were handed a favourable draw as the draws of the FA Trophy Third Round and Fourth down were held at the Trophies Lounge on Monday.
The Citizens, who beat Birkirkara in last season’s final to complete a memorable league and cup double, were drawn to face Division One strugglers Marsa.
Should they overcome Marsa, Valletta will face the winner of the tie between Mellieħa and Lija Athletic.
Birkirara, on the other hand, were handed a tricky start to the cup competition when they were drawn against Gozitan side Nadur Youngsters.
The third round draw provided four all Premier League ties with the plum fixture being the clash between Floriana and Ħamrun Spartans.
The winners of this clash will face the winner of the match between Birkirkara and Nadur.
Sliema Wanderers were drawn to face fellow Premier League side St Andrews while Hibernians will be up against Qormi.
The other tie featuring top division clubs puts against each other Mosta and Senglea Athletic
Gzira United are favourites to reach the fourth round when they face second division side Rabat Ajax while Balzan will play Qrendi.
Third round draw
Floriana vs Hamrun Spartans
Hibernians vs Qormi
Balzan vs Qrendi
Nadur Youngsters vs Birkirkara vs
Gzira United vs Rabat Ajax
Valletta vs Marsa
Mellieħa vs Lija Athletic
San Ġwann vs Fgura United
Gudja United vs Xewkija Tigers
Pembroke Athleta vs Żejtun Corinthians
Sliema Wanderers vs St Andrews
Vittoriosa Stars vs Tarxien Rainbows
Senglea Athletic vs Mosta
Siġġiewi vs Melita
Birżebbuġa St Peter’s vs Żebbuġ Rangers
Mdina Knights vs Pieta Hotspurs
Matches to be played November 30 and December 2.
Fourth round draw
Gżira/Rabat vs Siġġiewi/Melita
Mellieħa/Lija vs Valletta/Marsa
Senglea/Mosta vs Sliema/St Andrews
Mdina/Pieta vs Pembroke/Żejtuna
Nadur/Birkirkara vs Floriana/Ħamrun
Vittoriosa/Tarxien vs Birżebbuġa/Żebbug
Hibernians/Qormi vs San Ġwann/Fgura
Balzan/Qrendi vs Gudja/Xewkija
Matches to be played January 26 and 27.